{"id":278,"date":"2011-02-28T14:46:25","date_gmt":"2011-02-28T14:46:25","guid":{"rendered":"http:\/\/blogs.truman.edu\/mathcs\/?p=278"},"modified":"2011-02-28T14:46:25","modified_gmt":"2011-02-28T14:46:25","slug":"math-bio-colloquium-on-thursday-at-330-in-mg2090","status":"publish","type":"post","link":"https:\/\/blogs.truman.edu\/mathcs\/2011\/02\/28\/math-bio-colloquium-on-thursday-at-330-in-mg2090\/","title":{"rendered":"Math Bio Colloquium on Thursday at 3:30 in MG2090"},"content":{"rendered":"<p>Dr. Janick-Buckner will be the speaker at the next mathbio colloquium. <\/p>\n<p>Abstract: Characterization of a New ROUGH SHEATH1 Mutant of Maize (Development &amp; Cell Biology)<br \/>\nGraves, M. Christine1; Tracy, Christopher1; Khidir, Hazar1; Scanlon, Michael2; Buckner, Brent1; Janick-Buckner, Diane1<br \/>\n1 Truman State University, Kirksville, MO, 63501<br \/>\n2 Cornell University, Ithaca, NY, 14853<\/p>\n<p>In maize, class 1 knox genes are expressed in the shoot apical meristem and encode transcription factors that are involved in cell fate decisions. A knox-like mutant was identified in a maize line that exhibits active Mutator transposition. This knox-like phenotype is evident as aberrant cell patterning at the leaf sheath\/blade boundary and develops on more developmentally mature plants. SSR mapping indicates that the gene responsible for this mutant phenotype is closely linked to rough sheath1 (rs1), a class 1 knox gene. In addition, a Mu-TIR\/rs1 PCR primer pair consistently amplified DNA in mutant plants. RT-PCR was used to qualitatively characterize the expression of eight class 1 knox genes in leaf tissues. rs1, lg3, knox5, knox8 and knox11 were all ectopically expressed in the mutant sheath\/blade boundary; rs1 was also ectopically expressed in mutant blade tissue. Preliminary histological analysis of this Mu-tagged rs1 mutant indicates that there may be an expansion of sclerenchyma cells in sheath\/blade boundary and that there is less lignin found in this region compared to wild-type plants. We also observed that these Mu-tagged rs1 plants have abundant lesions on their leaf blades, which appears to be suppressed when plants also bear a Mu-tagged WRKY1 allele. Current studies continue to investigate the histological and lignin content of plants segregating for Mu-tagged rs1 and\/or Mu-tagged WKRY1 alleles.<\/p>\n<p>Funding acknowledgement: National Science Foundation (NSF)<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Dr. Janick-Buckner will be the speaker at the next mathbio colloquium. Abstract: Characterization of a New ROUGH SHEATH1 Mutant of Maize (Development &amp; Cell Biology) Graves, M. Christine1; Tracy, Christopher1; Khidir, Hazar1; Scanlon, Michael2; Buckner, Brent1; Janick-Buckner, Diane1 1 Truman State University, Kirksville, MO, 63501 2 Cornell University, Ithaca, NY, 14853 In maize, class 1 [&hellip;]<\/p>\n","protected":false},"author":185,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[1],"tags":[],"class_list":["post-278","post","type-post","status-publish","format-standard","hentry","category-uncategorized"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.7 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Math Bio Colloquium on Thursday at 3:30 in MG2090 - Math &amp; CS<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/blogs.truman.edu\/mathcs\/2011\/02\/28\/math-bio-colloquium-on-thursday-at-330-in-mg2090\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Math Bio Colloquium on Thursday at 3:30 in MG2090 - Math &amp; CS\" \/>\n<meta property=\"og:description\" content=\"Dr. Janick-Buckner will be the speaker at the next mathbio colloquium. 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