Dr. Janick-Buckner will be the speaker at the next mathbio colloquium. <\/p>\n
Abstract: Characterization of a New ROUGH SHEATH1 Mutant of Maize (Development & Cell Biology)
\nGraves, M. Christine1; Tracy, Christopher1; Khidir, Hazar1; Scanlon, Michael2; Buckner, Brent1; Janick-Buckner, Diane1
\n1 Truman State University, Kirksville, MO, 63501
\n2 Cornell University, Ithaca, NY, 14853<\/p>\n
In maize, class 1 knox genes are expressed in the shoot apical meristem and encode transcription factors that are involved in cell fate decisions. A knox-like mutant was identified in a maize line that exhibits active Mutator transposition. This knox-like phenotype is evident as aberrant cell patterning at the leaf sheath\/blade boundary and develops on more developmentally mature plants. SSR mapping indicates that the gene responsible for this mutant phenotype is closely linked to rough sheath1 (rs1), a class 1 knox gene. In addition, a Mu-TIR\/rs1 PCR primer pair consistently amplified DNA in mutant plants. RT-PCR was used to qualitatively characterize the expression of eight class 1 knox genes in leaf tissues. rs1, lg3, knox5, knox8 and knox11 were all ectopically expressed in the mutant sheath\/blade boundary; rs1 was also ectopically expressed in mutant blade tissue. Preliminary histological analysis of this Mu-tagged rs1 mutant indicates that there may be an expansion of sclerenchyma cells in sheath\/blade boundary and that there is less lignin found in this region compared to wild-type plants. We also observed that these Mu-tagged rs1 plants have abundant lesions on their leaf blades, which appears to be suppressed when plants also bear a Mu-tagged WRKY1 allele. Current studies continue to investigate the histological and lignin content of plants segregating for Mu-tagged rs1 and\/or Mu-tagged WKRY1 alleles.<\/p>\n
Funding acknowledgement: National Science Foundation (NSF)<\/p>\n","protected":false},"excerpt":{"rendered":"
Dr. Janick-Buckner will be the speaker at the next mathbio colloquium. Abstract: Characterization of a New ROUGH SHEATH1 Mutant of Maize (Development & Cell Biology) Graves, M. Christine1; Tracy, Christopher1; Khidir, Hazar1; Scanlon, Michael2; Buckner, Brent1; Janick-Buckner, Diane1 1 Truman State University, Kirksville, MO, 63501 2 Cornell University, Ithaca, NY, 14853 In maize, class 1 […]<\/p>\n","protected":false},"author":185,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[1],"tags":[],"class_list":["post-278","post","type-post","status-publish","format-standard","hentry","category-uncategorized"],"acf":[],"_links":{"self":[{"href":"https:\/\/blogs.truman.edu\/mathcs\/wp-json\/wp\/v2\/posts\/278","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/blogs.truman.edu\/mathcs\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/blogs.truman.edu\/mathcs\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/blogs.truman.edu\/mathcs\/wp-json\/wp\/v2\/users\/185"}],"replies":[{"embeddable":true,"href":"https:\/\/blogs.truman.edu\/mathcs\/wp-json\/wp\/v2\/comments?post=278"}],"version-history":[{"count":1,"href":"https:\/\/blogs.truman.edu\/mathcs\/wp-json\/wp\/v2\/posts\/278\/revisions"}],"predecessor-version":[{"id":279,"href":"https:\/\/blogs.truman.edu\/mathcs\/wp-json\/wp\/v2\/posts\/278\/revisions\/279"}],"wp:attachment":[{"href":"https:\/\/blogs.truman.edu\/mathcs\/wp-json\/wp\/v2\/media?parent=278"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/blogs.truman.edu\/mathcs\/wp-json\/wp\/v2\/categories?post=278"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/blogs.truman.edu\/mathcs\/wp-json\/wp\/v2\/tags?post=278"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}